Coding
Part:BBa_K3237002:Design
Designed by: Aubrey Demchuk, Kristi Turton Group: iGEM19_Lethbridge (2019-10-05)
His-mRFP1 codon optimized for C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 363
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
After the N-terminal histag we also have added a GC linker so that the his tag addition will not affect the protein structure of mRFP1.
Source
The part is based on the part BBa_E1010 in the iGEM registry. The part was codon optimized and then synthesized by TWIST.